human gamma secretase Search Results


human gamma secretase, gs genlisa elisa  (Krishgen Biosystems)
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ELISA for estimation of Human Gamma Secretase, GS in serum, plasma and other biological fluids
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human gamma-secretase activating protein  (Shanghai Korain Biotech Co Ltd)
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Regulator of gamma-secretase activity, which specifically activates the production of amyloid-beta protein (amyloid-beta protein 40 and amyloid-beta protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an
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human secretase elisa kit  (Cusabio)
92
Cusabio human secretase elisa kit
Fig. 2. Effect of PrPC on the expression of APP, BACE1, A40, sAPP, and sAPP. A) PrPC overexpressing HEK293 cells were analyzed 24 and 48 h after transfection by western blotting in comparison to control cells (1). Quantification of the band-intensity showed that PrPC was approximately 2-fold upregulated 24 h- and approximately 3.5 fold upregulated 48 h after transfection (1). Treatment with PNGase resulted in a deglycosylation of PrPC to prove the specificity of the PrPC detection (2). Concentration of PrPC was determined 48 h after transfection using a PrP <t>ELISA-assay</t> (3). B) Expression of APP and BACE1 was analyzed 24 and 48 h after transfection by western blotting. Both proteins were not influenced by the expression of PrPC. C) Amount of APP-cleavage fragments were measured via ELISA. Intra- and extracellular levels of A40, sAPP, and sAPP were decreased significantly in PrPC over-expressing cells (1–3). All in vitro experiments were carried out at least in triplicates. D) In mice, analysis of APP and BACE1 expression revealed no differences between Prnp0/0 and WT mice. E) ELISA measurement of A40 generation in mice brains revealed a marked, age-independent increase in Prnp0/0 mice compared to WT animals (1). PrPC overexpressing Tg35 mice revealed a decreased level of A40 (2) whereas concentration of <t>-secretase</t> remained unchanged in Prnp0/0 mice (3). All values were calculated in % of WT. Beta actin was used as an internal standard. For comparison between groups we used the student’s t-test (n = 5 per group in three independent experiments). Error bars indicate standard errors of the mean (SEM).
Human Secretase Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+gamma+secretase/Human+%CE%B3-Secretase+ELISA+kit/10__3233_slash_jad___130566-119-4-8
Average 92 stars, based on 1 article reviews
human secretase elisa kit - by Bioz Stars, 2026-09
92/100 stars
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recombinant human gamma-secretase subunit pen-2  (Cusabio)
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human gamma-secretase subunit aph-1a  (Shanghai Korain Biotech Co Ltd)
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Non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed: 12297508, PubMed: 12522139, PubMed: 12763021, PubMed: 12679784,
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human gamma-secretase activating protein, gsap genlisa elisa  (Krishgen Biosystems)
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ELISA for estimation of Human Gamma-Secretase Activating Protein, gSAP in serum, plasma and other biological fluids
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human gamma-secretase subunit aph-1a, aph1a genlisa elisa  (Krishgen Biosystems)
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ELISA for estimation of Human Gamma-secretase subunit APH-1A, APH1A in serum, plasma and other biological fluids
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recombinant human gamma-secretase subunit aph-1a(aph1a),partial  (Cusabio)
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Image Search Results


Fig. 2. Effect of PrPC on the expression of APP, BACE1, A40, sAPP, and sAPP. A) PrPC overexpressing HEK293 cells were analyzed 24 and 48 h after transfection by western blotting in comparison to control cells (1). Quantification of the band-intensity showed that PrPC was approximately 2-fold upregulated 24 h- and approximately 3.5 fold upregulated 48 h after transfection (1). Treatment with PNGase resulted in a deglycosylation of PrPC to prove the specificity of the PrPC detection (2). Concentration of PrPC was determined 48 h after transfection using a PrP ELISA-assay (3). B) Expression of APP and BACE1 was analyzed 24 and 48 h after transfection by western blotting. Both proteins were not influenced by the expression of PrPC. C) Amount of APP-cleavage fragments were measured via ELISA. Intra- and extracellular levels of A40, sAPP, and sAPP were decreased significantly in PrPC over-expressing cells (1–3). All in vitro experiments were carried out at least in triplicates. D) In mice, analysis of APP and BACE1 expression revealed no differences between Prnp0/0 and WT mice. E) ELISA measurement of A40 generation in mice brains revealed a marked, age-independent increase in Prnp0/0 mice compared to WT animals (1). PrPC overexpressing Tg35 mice revealed a decreased level of A40 (2) whereas concentration of -secretase remained unchanged in Prnp0/0 mice (3). All values were calculated in % of WT. Beta actin was used as an internal standard. For comparison between groups we used the student’s t-test (n = 5 per group in three independent experiments). Error bars indicate standard errors of the mean (SEM).

Journal: Journal of Alzheimer's Disease

Article Title: Impact of the Cellular Prion Protein on Amyloid-β and 3PO-Tau Processing

doi: 10.3233/jad-130566

Figure Lengend Snippet: Fig. 2. Effect of PrPC on the expression of APP, BACE1, A40, sAPP, and sAPP. A) PrPC overexpressing HEK293 cells were analyzed 24 and 48 h after transfection by western blotting in comparison to control cells (1). Quantification of the band-intensity showed that PrPC was approximately 2-fold upregulated 24 h- and approximately 3.5 fold upregulated 48 h after transfection (1). Treatment with PNGase resulted in a deglycosylation of PrPC to prove the specificity of the PrPC detection (2). Concentration of PrPC was determined 48 h after transfection using a PrP ELISA-assay (3). B) Expression of APP and BACE1 was analyzed 24 and 48 h after transfection by western blotting. Both proteins were not influenced by the expression of PrPC. C) Amount of APP-cleavage fragments were measured via ELISA. Intra- and extracellular levels of A40, sAPP, and sAPP were decreased significantly in PrPC over-expressing cells (1–3). All in vitro experiments were carried out at least in triplicates. D) In mice, analysis of APP and BACE1 expression revealed no differences between Prnp0/0 and WT mice. E) ELISA measurement of A40 generation in mice brains revealed a marked, age-independent increase in Prnp0/0 mice compared to WT animals (1). PrPC overexpressing Tg35 mice revealed a decreased level of A40 (2) whereas concentration of -secretase remained unchanged in Prnp0/0 mice (3). All values were calculated in % of WT. Beta actin was used as an internal standard. For comparison between groups we used the student’s t-test (n = 5 per group in three independent experiments). Error bars indicate standard errors of the mean (SEM).

Article Snippet: We used a commercial Human -Secretase ELISA Kit (Cusabio, Wuhan, China) to analyze brain homogenates derived from Prnp0/0 and WT mice.

Techniques: Expressing, Transfection, Western Blot, Comparison, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, In Vitro